Detection of carbapenem-resistance and biofilm formation genes, and genetic relatedness of Acinetobacter baumannii isolates

Acinetobacter baumannii is one of the most important nosocomial pathogen worldwide. This study aimed to investigate virulence potential and genomic relatedness A. strains isolated from patients hospitalized in Military Medical Academy (MMA) by detecting OXA-type carbapenemases genes, biofilm-associated RAPD analysis. PCR was used detect blaoxa ISAba-1 genetic element, genes. The determined analysis using four different primers (AP2, DAF4. M13, DECA). blaoxa-51-like, blaoxa-23-like, blaoxa-24-like, blaoxa-58-like were present 100%, 34.0%, 62.4%, 3.1% isolates, respectively. All isolates had ISAba1 sequence their genome, 35.1% it associated with 97.0% blaoxa-23-like gene. Biofilm-associated genes bap, ompA, epsA, csuA/BABCDE, pgaABCD detected 93.8%, 95.8%, 88.1%, 98.4%, 98.9% showed a high degree genome similarity clonal dispersion isolates. Detection especially percentage indicated great pathogenic potential. revealed level majority through MMA. Further, continuous introduction individual profiles contributes diversity These results can be useful for further management tracking outbreaks.